Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei     

于洋  张晓军  刘敬文  李富花  黄皓  李义军  刘小林  相建海 《中国海洋湖沼学报》2015,33(1):1-10

Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance.However,the identification of new varieties of shrimps from their phenotypic characters is difficult.This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences.The method was employed to identify a new selected variety,Kehai No.1(KH-1),from three representative stocks(control group):Zhengda;Tongwei;and a stock collected from Fujian Province,which is now cultured in mainland China.By pooled genotyping of KH-1 and the control group,five microsatellites showing differences between KH-1 and the control group were screened out.Individual genotyping data confirmed the results from pooled genotyping.The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2.By sequencing the mitochondrial control regions of individuals from the KH-1 and control group,four haplotypes were observed in the KH-1 group,whereas14 haplotypes were obtained in the control group.By combining the microsatellite assignment analysis with mitochondrial control region analysis,the average accuracy of identification of individuals in the KH-1group and control group reached 89%.The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L.vannamei from other populations cultured in China.  相似文献   

Impact of nonlinear processes on formation of the Kuroshio large meander path in a barotropic inflow-outflow model     

张培军  王强  马利斌 《中国海洋湖沼学报》2015,33(1):252-261

Based on a barotropic inflow-outflow model,we examine the formation of the Kuroshio large meander(LM) using conditional nonlinear optimal perturbation(CNOP) method.Both linear and nonlinear evolutions of such perturbations obtained by this method are investigated.The results show that the nonlinear evolution can result in the Kuroshio transition from a straight to LM path,whereas the linear evolution cannot.This implies that nonlinearity plays an important role in the formation of the Kuroshio LM path.The nonlinearity exists as advection in the evolution equations of the perturbation derived from the barotropic inflow-outflow model,namely the nonlinear advection of the perturbation by the perturbation(NAPP).By examining the role of this nonlinearity,we find that the NAPP tends to move the cyclonic eddy induced by the CNOP-type perturbation westward.Together with the beta effect,this offsets part of the eastward advection caused by the interaction between the perturbation and the background flow.Hence,the eastward movement of the cyclonic eddy is significantly weakened,effectively causing the eddy to develop.The sufficient evolution of this cyclonic eddy leads to the formation of the Kuroshio LM.  相似文献   

Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate     

王雪芹  邢荣娥  刘松  于华华  李克成  陈作元  李鹏程 《中国海洋湖沼学报》2015,33(1):159-168

Mackerel(Pneumatophorus japonicus) proteins were hydrolyzed by five proteases:trypsin,papain,neutrase,acid protease,and flavourzyme.The hydrolysate treated by neutrase exhibited the highest antioxidant activity.Response surface methodology(RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate(MPH) with the highest DPPH radical scavenging activity.The MPH was fractioned using a series of ultrafiltration membranes and five fractions,namely,MPH-Ⅰ(10kDa),MPH-Ⅱ(10-2.5 kDa),MPH-Ⅲ(1-2.5 kDa),MPH-Ⅳ(0.4-1 kDa),and MPH-V(below 0.4 kDa),were obtained.DPPH radical scavenging activity,reducing power,hydroxyl radical scavenging activity,and the lipid peroxidation inhibition capability of these fractions were evaluated.The fractions in molecular weights 2.5 kDa(MPH-Ⅲ,MPH-Ⅳ,and MPH-Ⅴ),which occupied 93.4%of the total fractions,showed the strongest antioxidant activity;and the antioxidant activities of the three fractions are similar to each other.Using SP Sephadex C-25 and Sephadex G-25 columns,eight fractions were obtained from the MPH(2.5 kDa).The isolated peptide I(1 664 kDa) displayed the highest DPPH radical scavenging activity.Therefore,MPH is a potential source of antioxidant peptides.  相似文献   

Monitoring cyanobacteria-dominant algal blooms in eutrophicated Taihu Lake in China with synthetic aperture radar images     

王甘霖  李俊生  张兵  申茜  张方方 《中国海洋湖沼学报》2015,33(1):139-148

Monitoring algal blooms by optical remote sensing is limited by cloud cover.In this study,synthetic aperture radar(SAR) was deployed with the aim of monitoring cyanobacteria-dominant algal blooms in Taihu Lake in cloudy weather.The study shows that dark regions in the SAR images caused by cyanobacterial blooms damped the microwave backscatter of the lake surface and were consistent with the regions of algal blooms in quasi-synchronous optical images,confirming the applicability of SAR for detection of surface blooms.Low backscatter may also be associated with other factors such as low wind speeds,resulting in interference when monitoring algal blooms using SAR data alone.After feature extraction and selection,the dark regions were classified by the support vector machine method with an overall accuracy of 67.74%.SAR can provide a reference point for monitoring cyanobacterial blooms in the lake,particularly when weather is not suitable for optical remote sensing.Multi-polarization and multi-band SAR can be considered for use in the future to obtain more accurate information regarding algal blooms from SAR data.  相似文献   

A theoretical framework and methodology for urban activity spatial structure in e-society: Empirical evidence for Nanjing City,China   总被引：7，自引：0，他引：7  

Bo Wang  Feng Zhen  Zongcai Wei  Shu Guo  Tingting Chen 《中国地理科学(英文版)》2015,25(6):672-683

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The burial of biogenic silica,organic carbon and organic nitrogen in the sediments of the East China Sea     

Wang  Lisha  Zhang  Chuansong  Shi  Xiaoyong 《中国海洋大学学报(英文版)》2015,14(3):464-470

We sampled the sediments of the East China Sea during 2005 and 2006, and analysed the contents of the biogenic mat-ters: biogenic silica, organic carbon, and organic nitrogen. From the surface distribu...  相似文献   

Sustainability evaluation of different systems for sea cucumber (Apostichopus japonicus) farming based on emergy theory     

Wang  Guodong  Dong  Shuanglin  Tian  Xiangli  Gao  Qinfeng  Wang  Fang 《中国海洋大学学报(英文版)》2015,14(3):503-510

Emergy analysis is effective for analyzing ecological economic systems. However, the accuracy of the approach is af-fected by the diversity of economic level, meteorological and hydrological parameters...  相似文献   

页岩气资源调查评价中的采样测试工作     

李昭容  梁晓玲  王志文 《国土资源导刊(湖南)》2015,(1):90-95

页岩气是一种新的能源矿产,具有巨大的勘查开发潜力,现已成为全球油气勘探的一个新领域。采样测试作为一种勘查手段,在页岩气气藏岩性、成因来源、赋存形式、成藏特征、成藏条件、含气量等方面的研究中起着极其重要的作用,在页岩气的勘查开发中具有极其重要的意义。在页岩气资源评价等勘查中,正确采集测试样品、合理选定测试项目和测试方法、有效运用采样测试这一勘查手段,是页岩气资源调查评价中一项十分重要的工作。  相似文献   

湖南省醴陵市金宏金矿成矿规律及找矿     

王淑军 《国土资源导刊(湖南)》2015,(2):30-33,38

通过对湘东北金宏金矿控矿因素、成矿规律、找矿方向等进行的野外调查和室内分析,表明:(1)中元古界冷家溪雷神庙组(Pt2l)为矿源层;(2)北西西向压扭性剪切断裂带为该区金矿的主要控矿构造;北北东向压扭性剪切断裂带是该区金矿的次要控矿构造;(3)该两组压扭性剪切断裂构造交汇部位是金矿富集最有利部位,很容易形成品位较高、厚度较大的矿床;该矿区矿床远景规模可达大型。  相似文献   

The mechanics of continental extension in Qiongdongnan Basin,northern South China Sea     

Zhongxian Zhao  Zhen Sun  Zhenfeng Wang  Zhipeng Sun 《Marine Geophysical Researches》2015,36(2-3):197-210

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